Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: MicroRNA-150 relieves vascular remodeling and fibrosis in hypoxia-induced pulmonary hypertension.

doi: 10.1016/j.biopha.2018.11.058

Figure Lengend Snippet: Fig. 4. Effect of miR-150 on pulmonary fibrosis of pulmonary hypertension rats. (A) Pulmonary fibrosis was detected by Masson’s staining (200× magnification). Scale bars, 100 μm. (B) The area of pulmonary fibrosis was calculated and shown. The mRNA expressions of TGF-β1 (C) and collagen I (D) in lung tissues were evaluated by qPCR. (E) The protein levels of TGF-β1 and collagen I in lung tissues were measured by western blot assay. (F)&(G) Relative grey values of the protein bands were shown. (H) The expressions of TGF-β1 and collagen I in lung tissues were detected by immunohistochemical staining (400× magnification). Scale bars, 50 μm. Data were presented as mean ± SD. **P < 0.01, ***P < 0.001 versus the indicated group.

Article Snippet: The membranes were blocked in 5% nonfat milk for 1 h at room temperature, then incubated with primary antibodies against TGF-β1 (1:400, BOSTER, China), Collagen I (1:300, BOSTER, China), p-AKTser473 (1:500, KeyGen, China), AKT (1:500, KeyGen, China), p-mTORser2481 (1:500, Sangon Biotech, China), mTOR (1:1000, Cell signaling Technology, USA), and β-actin (1:500, Bioss, China) at 4°C overnight, followed by incubation with HRP-labeled Goat AntiRabbit or Goat Anti-Mouse IgG (1:5000, Beyotime, China) at 37°C for 45min.

Techniques: Staining, Western Blot, Immunohistochemical staining

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: MicroRNA-150 relieves vascular remodeling and fibrosis in hypoxia-induced pulmonary hypertension.

doi: 10.1016/j.biopha.2018.11.058

Figure Lengend Snippet: Fig. 6. Effect of miR-150 on the expressions of fibrosis-related molecules. The mRNA expressions of TGF-β1 (A) and collagen I (B) in PASMCs were measured by qPCR. (C) The protein levels of TGF-β1 and collagen I in PASMCs were detected by western blot assay. (D)&(E) Relative grey values of the protein bands were shown. Data were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 versus the indicated group.

Article Snippet: The membranes were blocked in 5% nonfat milk for 1 h at room temperature, then incubated with primary antibodies against TGF-β1 (1:400, BOSTER, China), Collagen I (1:300, BOSTER, China), p-AKTser473 (1:500, KeyGen, China), AKT (1:500, KeyGen, China), p-mTORser2481 (1:500, Sangon Biotech, China), mTOR (1:1000, Cell signaling Technology, USA), and β-actin (1:500, Bioss, China) at 4°C overnight, followed by incubation with HRP-labeled Goat AntiRabbit or Goat Anti-Mouse IgG (1:5000, Beyotime, China) at 37°C for 45min.

Techniques: Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Traditional Chinese Medicine Gedan Jiangya Decoction Alleviates Left Ventricular Hypertrophy via Suppressing the Ras/ERK1/2 Signaling Pathway

doi: 10.1155/2022/6924197

Figure Lengend Snippet: The effect of GJD on left ventricular mass index in each group and serum TGF-beta 1 in each group. (a) Left ventricular mass index. (b) Serum TGF-beta 1 ( n = 7). Values are means ± SD. Control: WKY (Wistar–Kyoto rats), model: spontaneously hypertensive rats (SHRs), GJD-L: SHR received GJD low dose, GJD-M: SHR received GJD medium dose, GJD-H: SHR received GJD high dose, and CAPT: SHR received captopril. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. control. # P < 0.05, ## P < 0.01, and ### P < 0.001 vs. model.

Article Snippet: Primary antibodies include: TGF-beta 1 (bsm-33287m, 1 : 1,000, Bioss, China), Ras 1 (GB11411, 1 : 1,000, Servicebio, China), ERK1/2 (GB11560, 1 : 1,000, Servicebio, China), C-Fos (GB114125, 1 : 1,000, Servicebio, China), and β -actin (GB15001, 1 : 2,000, Servicebio, China).

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Traditional Chinese Medicine Gedan Jiangya Decoction Alleviates Left Ventricular Hypertrophy via Suppressing the Ras/ERK1/2 Signaling Pathway

doi: 10.1155/2022/6924197

Figure Lengend Snippet: GJD's effect on Ras/ERK1/2 signaling mRNA expression in the left ventricular tissue. (a) mRNA expression of TGF-beta 1, (b) mRNA expression of Ras, (c) mRNA expression of ERK1/2, and (d) mRNA expression of C-Fos ( n = 3). Values are means ± SD. Control: WKY (Wistar–Kyoto rats), model: spontaneously hypertensive rats (SHRs), GJD-L: SHR received GJD low dose, GJD-M: SHR received GJD medium dose, GJD-H: SHR received GJD high dose, and CAPT: SHR received captopril. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. control. # P < 0.05, ## P < 0.01, and ### P < 0.001 vs. model.

Article Snippet: Primary antibodies include: TGF-beta 1 (bsm-33287m, 1 : 1,000, Bioss, China), Ras 1 (GB11411, 1 : 1,000, Servicebio, China), ERK1/2 (GB11560, 1 : 1,000, Servicebio, China), C-Fos (GB114125, 1 : 1,000, Servicebio, China), and β -actin (GB15001, 1 : 2,000, Servicebio, China).

Techniques: Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Traditional Chinese Medicine Gedan Jiangya Decoction Alleviates Left Ventricular Hypertrophy via Suppressing the Ras/ERK1/2 Signaling Pathway

doi: 10.1155/2022/6924197

Figure Lengend Snippet: GJD's effect on the protein expression of Ras/ERK1/2 pathway of TGF-beta 1, Ras, ERK1/2, and C-Fos in left ventricular tissue in each group. (a) Expression level of TGF-beta1, Ras, ERK1/2, and C-Fos, (b) expression level of TGF-beta 1, (c) expression level of Ras, (d) expression level of ERK1/2, and (e) expression level of C-Fos ( n = 3). Values are means ± SD. Control: WKY (Wistar–Kyoto rats), model: spontaneously hypertensive rats (SHRs), GJD-L: SHR received GJD low dose, GJD-M: SHR received GJD medium dose, GJD-H: SHR received GJD high dose, and CAPT: SHR received captopril. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. control. # P < 0.05, ## P < 0.01, and ### P < 0.001 vs. model.

Article Snippet: Primary antibodies include: TGF-beta 1 (bsm-33287m, 1 : 1,000, Bioss, China), Ras 1 (GB11411, 1 : 1,000, Servicebio, China), ERK1/2 (GB11560, 1 : 1,000, Servicebio, China), C-Fos (GB114125, 1 : 1,000, Servicebio, China), and β -actin (GB15001, 1 : 2,000, Servicebio, China).

Techniques: Expressing